| 质粒名称: | pcDNA3.1(+)Flag-His-ATM wt |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 31985 |
| 存储实验室: | Michael Kastan |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5428 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | ATM wild-type,ATI,ATA,ATDC |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 9581 bp |
| 基因突变: | 3' UTR of 400 nucl. |
| GenBank编号 | NP_000042.3/71902540 |
| 启动子 | -- |
| 标签/融合蛋白: |
Flag（主干上的N终端）
HIS（主干N端）
|
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XhoI-SalI连接（在克隆过程中被破坏） |
| 3' 克隆位点: | XhoI（未销毁） |
| 5' 测序引物 | T7 |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA3.1(+)Flag-His-ATM wt was a gift from Michael Kastan (Addgene plasmid # 31985 ; http://n2t.net/addgene:31985 ; RRID:Addgene_31985) |
| 参考文献部分: | Activation of the ATM kinase by ionizing radiation and phosphorylation of p53. Canman CE, Lim DS, Cimprich KA, Taya Y, Tamai K, Sakaguchi K, Appella E, Kastan MB, Siliciano JD. Science. 1998 Sep 11;281(5383):1677-9. 10.1126/science.281.5383.1677 PubMed 9733515 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
Flag-His-ATM wt质粒图谱.png)
载体简介
载体序列
LOCUS pcDNA3.1(+)Flag-His-ATM wt 15084 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION Mammalian expression of human ATM with Flag and His tags. ACCESSION . VERSION . KEYWORDS pcDNA3.1(+)Flag-His-ATM wt SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 15084) AUTHORS Canman CE, Lim DS, Cimprich KA, Taya Y, Tamai K, Sakaguchi K, Appella E, Kastan MB, Siliciano JD TITLE Activation of the ATM kinase by ionizing radiation and phosphorylation of p53. JOURNAL Science. 1998 Sep 11;281(5383):1677-9. PUBMED 9733515 REFERENCE 2 (bases 1 to 15084) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..15084 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 984..1007 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 1011..1028 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" promoter complement(10637..10655) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(10638..10655) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(10675..10692) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 10681..10905 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 10951..11379 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(11038..11057) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 11248..11269 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(11388..11408) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 11393..11722 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 11573..11708 /label=SV40 ori /note="SV40 origin of replication" primer_bind 11635..11654 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 11789..12583 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(11843..11862) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 12453..12472 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 12757..12878 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(12794..12813) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 12848..12867 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(12927..12943) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(12927..12943) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(12940..12962) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 12951..12967 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(12975..13005) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 13020..13041 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(13158..13175) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(13329..13917) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(13409..13428) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(14088..14948) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 14711..14730 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(14949..15053) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgcagat atccatcaca 961 ctggcggccg ctcgaccacc atggactaca aggacgatga cgacaagatg catcatcatc 1021 atcatcatat gagtctagta cttaatgatc tgcttatctg ctgccgtcaa ctagaacatg 1081 atagagctac agaacgaaag aaagaagttg agaaatttaa gcgcctgatt cgagatcctg 1141 aaacaattaa acatctagat cggcattcag attccaaaca aggaaaatat ttgaattggg 1201 atgctgtttt tagattttta cagaaatata ttcagaaaga aacagaatgt ctgagaatag 1261 caaaaccaaa tgtatcagcc tcaacacaag cctccaggca gaaaaagatg caggaaatca 1321 gtagtttggt caaatacttc atcaaatgtg caaacagaag agcacctagg ctaaaatgtc 1381 aagaactctt aaattatatc atggatacag tgaaagattc atctaatggt gctatttacg 1441 gagctgattg tagcaacata ctactcaaag acattctttc tgtgagaaaa tactggtgtg 1501 aaatatctca gcaacagtgg ttagaattgt tctctgtgta cttcaggctc tatctgaaac 1561 cttcacaaga tgttcataga gttttagtgg ctagaataat tcatgctgtt accaaaggat 1621 gctgttctca gactgacgga ttaaattcca aatttttgga ctttttttcc aaggctattc 1681 agtgtgcgag acaagaaaag agctcttcag gtctaaatca tatcttagca gctcttacta 1741 tcttcctcaa gactttggct gtcaactttc gaattcgagt gtgtgaatta ggagatgaaa 1801 ttcttcccac tttgctttat atttggactc aacataggct taatgattct ttaaaagaag 1861 tcattattga attatttcaa ctgcaaattt atatccatca tccgaaagga gccaaaaccc 1921 aagaaaaagg tgcttatgaa tcaacaaaat ggagaagtat tttatacaac ttatatgatc 1981 tgctagtgaa tgagataagt catataggaa gtagaggaaa gtattcttca ggatttcgta 2041 atattgccgt caaagaaaat ttgattgaat tgatggcaga tatctgtcac caggttttta 2101 atgaagatac cagatccttg gagatttctc aatcttacac tactacacaa agagaatcta 2161 gtgattacag tgtcccttgc aaaaggaaga aaatagaact aggctgggaa gtaataaaag 2221 atcaccttca gaagtcacag aatgattttg atcttgtgcc ttggctacag attgcaaccc 2281 aattaatatc aaagtatcct gcaagtttac ctaactgtga gctgtctcca ttactgatga 2341 tactatctca gcttctaccc caacagcgac atggggaacg tacaccatat gtgttacgat 2401 gccttacgga agttgcattg tgtcaagaca agaggtcaaa cctagaaagc tcacaaaagt 2461 cagatttatt aaaactctgg aataaaattt ggtgtattac ctttcgtggt ataagttctg 2521 agcaaataca agctgaaaac tttggcttac ttggagccat aattcagggt agtttagttg 2581 aggttgacag agaattctgg aagttattta ctgggtcagc ctgcagacct tcatgtcctg 2641 cagtatgctg tttgactttg gcactgacca ccagtatagt tccaggaacg gtaaaaatgg 2701 gaatagagca aaatatgtgt gaagtaaata gaagcttttc tttaaaggaa tcaataatga 2761 aatggctctt attctatcag ttagagggtg acttagaaaa tagcacagaa gtgcctccaa 2821 ttcttcacag taattttcct catcttgtac tggagaaaat tcttgtgagt ctcactatga 2881 aaaactgtaa agctgcaatg aattttttcc aaagcgtgcc agagtgtgaa caccaccaaa 2941 aagataaaga agaactttca ttctcagaag tagaagaact atttcttcag acaacttttg 3001 acaagatgga ctttttaacc attgtgagag aatgtggtat agaaaagcac cagtccagta 3061 ttggcttctc tgtccaccag aatctcaagg aatcactgga tcgctgtctt ctgggattat 3121 cagaacagct tctgaataat tactcatctg agattacaaa ttcagaaact cttgtccggt 3181 gttcacgtct tttggtgggt gtccttggct gctactgtta catgggtgta atagctgaag 3241 aggaagcata taagtcagaa ttattccaga aagccaagtc tctaatgcaa tgtgcaggag 3301 aaagtatcac tctgtttaaa aataagacaa atgaggaatt cagaattggt tccttgagaa 3361 atatgatgca gctatgtaca cgttgcttga gcaactgtac caagaagagt ccaaataaga 3421 ttgcatctgg ctttttcctg cgattgttaa catcaaagct aatgaatgac attgcagata 3481 tttgtaaaag tttagcatcc ttcatcaaaa agccatttga ccgtggagaa gtagaatcaa 3541 tggaagatga tactaatgga aatctaatgg aggtggagga tcagtcatcc atgaatctat 3601 ttaacgatta ccctgatagt agtgttagtg atgcaaacga acctggagag agccaaagta 3661 ccataggtgc cattaatcct ttagctgaag aatatctgtc aaagcaagat ctacttttct 3721 tagacatgct caagttcttg tgtttgtgtg taactactgc tcagaccaat actgtgtcct 3781 ttagggcagc tgatattcgg aggaaattgt taatgttaat tgattctagc acgctagaac 3841 ctaccaaatc cctccacctg catatgtatc taatgctttt aaaggagctt cctggagaag 3901 agtacccctt gccaatggaa gatgttcttg aacttctgaa accactatcc aatgtgtgtt 3961 ctttgtatcg tcgtgaccaa gatgtttgta aaactatttt aaaccatgtc cttcatgtag 4021 tgaaaaacct aggtcaaagc aatatggact ctgagaacac aagggatgct caaggacagt 4081 ttctaacagt aattggagca ttttggcatc taacaaagga gaggaaatat atattctctg 4141 taagaatggc cctagtaaat tgccttaaaa ctttgcttga ggctgatcct tattcaaaat 4201 gggccattct taatgtaatg ggaaaagact ttcctgtaaa tgaagtattt acacaatttc 4261 ttgctgacaa tcatcaccaa gttcgcatgt tggctgcaga gtcaatcaat agattgttcc 4321 aggacacgaa gggagattct tccaggttac tgaaagcact tcctttgaag cttcagcaaa 4381 cagcttttga aaatgcatac ttgaaagctc aggaaggaat gagagaaatg tcccatagtg 4441 ctgagaaccc tgaaactttg gatgaaattt ataatagaaa atctgtttta ctgacgttga 4501 tagctgtggt tttatcctgt agccctatct gcgaaaaaca ggctttgttt gccctgtgta 4561 aatctgtgaa agagaatgga ttagaacctc accttgtgaa aaaggtttta gagaaagttt 4621 ctgaaacttt tggatataga cgtttagaag actttatggc atctcattta gattatctgg 4681 ttttggaatg gctaaatctt caagatactg aatacaactt atcttctttt ccttttattt 4741 tattaaacta cacaaatatt gaggatttct atagatcttg ttataaggtt ttgattccac 4801 atctggtgat tagaagtcat tttgatgagg tgaagtccat tgctaatcag attcaagagg 4861 actggaaaag tcttctaaca gactgctttc caaagattct tgtaaatatt cttccttatt 4921 ttgcctatga gggtaccaga gacagtggga tggcacagca aagagagact gctaccaagg 4981 tctatgatat gcttaaaagt gaaaacttat tgggaaaaca gattgatcac ttattcatta 5041 gtaatttacc agagattgtg gtggagttat tgatgacgtt acatgagcca gcaaattcta 5101 gtgccagtca gagcactgac ctctgtgact tttcagggga tttggatcct gctcctaatc 5161 cacctcattt tccatcgcat gtgattaaag caacatttgc ctatatcagc aattgtcata 5221 aaaccaagtt aaaaagcatt ttagaaattc tttccaaaag ccctgattcc tatcagaaaa 5281 ttcttcttgc catatgtgag caagcagctg aaacaaataa tgtttataag aagcacagaa 5341 ttcttaaaat atatcacctg tttgttagtt tattactgaa agatataaaa agtggcttag 5401 gaggagcttg ggcctttgtt cttcgagacg ttatttatac tttgattcac tatatcaacc 5461 aaaggccttc ttgtatcatg gatgtgtcat tacgtagctt ctccctttgt tgtgacttat 5521 taagtcaggt ttgccagaca gccgtgactt actgtaagga tgctctagaa aaccatcttc 5581 atgttattgt tggtacactt ataccccttg tgtatgagca ggtggaggtt cagaaacagg 5641 tattggactt gttgaaatac ttagtgatag ataacaagga taatgaaaac ctctatatca 5701 cgattaagct tttagatcct tttcctgacc atgttgtttt taaggatttg cgtattactc 5761 agcaaaaaat caaatacagt agaggaccct tttcactctt ggaggaaatt aaccattttc 5821 tctcagtaag tgtttatgat gcacttccat tgacaagact tgaaggacta aaggatcttc 5881 gaagacaact ggaactacat aaagatcaga tggtggacat tatgagagct tctcaggata 5941 atccgcaaga tgggattatg gtgaaactag ttgtcaattt gttgcagtta tccaagatgg 6001 caataaacca cactggtgaa aaagaagttc tagaggctgt tggaagctgc ttgggagaag 6061 tgggtcctat agatttctct accatagcta tacaacatag taaagatgca tcttatacca 6121 aggcccttaa gttatttgaa gataaagaac ttcagtggac cttcataatg ctgacctacc 6181 tgaataacac actggtagaa gattgtgtca aagttcgatc agcagctgtt acctgtttga 6241 aaaacatttt agccacaaag actggacata gtttctggga gatttataag atgacaacag 6301 atccaatgct ggcctatcta cagcctttta gaacatcaag aaaaaagttt ttagaagtac 6361 ccagatttga caaagaaaac ccttttgaag gcctggatga tataaatctg tggattcctc 6421 taagtgaaaa tcatgacatt tggataaaga cactgacttg tgcttttttg gacagtggag 6481 gcacaaaatg tgaaattctt caattattaa agccaatgtg tgaagtgaaa actgactttt 6541 gtcagactgt acttccatac ttgattcatg atattttact ccaagataca aatgaatcat 6601 ggagaaatct gctttctaca catgttcagg gatttttcac cagctgtctt cgacacttct 6661 cgcaaacgag ccgatccaca acccctgcaa acttggattc agagtcagag cactttttcc 6721 gatgctgttt ggataaaaaa tcacaaagaa caatgcttgc tgttgtggac tacatgagaa 6781 gacaaaagag accttcttca ggaacaattt ttaatgatgc tttctggctg gatttaaatt 6841 atctagaagt tgccaaggta gctcagtctt gtgctgctca ctttacagct ttactctatg 6901 cagaaatcta tgcagataag aaaagtatgg atgatcaaga gaaaagaagt cttgcatttg 6961 aagaaggaag ccagagtaca actatttcta gcttgagtga aaaaagtaaa gaagaaactg 7021 gaataagttt acaggatctt ctcttagaaa tctacagaag tataggggag ccagatagtt 7081 tgtatggctg tggtggaggg aagatgttac aacccattac tagactacga acatatgaac 7141 acgaagcaat gtggggcaaa gccctagtaa catatgacct cgaaacagca atcccctcat 7201 caacacgcca ggcaggaatc attcaggcct tgcagaattt gggactctgc catattcttt 7261 ccgtctattt aaaaggattg gattatgaaa ataaagactg gtgtcctgaa ctagaagaac 7321 ttcattacca agcagcatgg aggaatatgc agtgggacca ttgcacttcc gtcagcaaag 7381 aagtagaagg aaccagttac catgaatcat tgtacaatgc tctacaatct ctaagagaca 7441 gagaattctc tacattttat gaaagtctca aatatgccag agtaaaagaa gtggaagaga 7501 tgtgtaagcg cagccttgag tctgtgtatt cgctctatcc cacacttagc aggttgcagg 7561 ccattggaga gctggaaagc attggggagc ttttctcaag atcagtcaca catagacaac 7621 tctctgaagt atatattaag tggcagaaac actcccagct tctcaaggac agtgatttta 7681 gttttcagga gcctatcatg gctctacgca cagtcatttt ggagatcctg atggaaaagg 7741 aaatggacaa ctcacaaaga gaatgtatta aggacattct caccaaacac cttgtagaac 7801 tctctatact ggccagaact ttcaagaaca ctcagctccc tgaaagggca atatttcaaa 7861 ttaaacagta caattcagtt agctgtggag tctctgagtg gcagctggaa gaagcacaag 7921 tattctgggc aaaaaaggag cagagtcttg ccctgagtat tctcaagcaa atgatcaaga 7981 agttggatgc cagctgtgca gcgaacaatc ccagcctaaa acttacatac acagaatgtc 8041 tgagggtttg tggcaactgg ttagcagaaa cgtgcttaga aaatcctgcg gtcatcatgc 8101 agacctatct agaaaaggca gtagaagttg ctggaaatta tgatggagaa agtagtgatg 8161 agctaagaaa tggaaaaatg aaggcatttc tctcattagc ccggttttca gatactcaat 8221 accaaagaat tgaaaactac atgaaatcat cggaatttga aaacaagcaa gctctcctga 8281 aaagagccaa agaggaagta ggtctcctta gggaacataa aattcagaca aacagataca 8341 cagtaaaggt tcagcgagag ctggagttgg atgaattagc cctgcgtgca ctgaaagagg 8401 atcgtaaacg cttcttatgt aaagcagttg aaaattatat caactgctta ttaagtggag 8461 aagaacatga tatgtgggta ttccgacttt gttccctctg gcttgaaaat tctggagttt 8521 ctgaagtcaa tggcatgatg aagagagacg gaatgaagat tccaacatat aaatttttgc 8581 ctcttatgta ccaattggct gctagaatgg ggaccaagat gatgggaggc ctaggatttc 8641 atgaagtcct caataatcta atctctagaa tttcaatgga tcacccccat cacactttgt 8701 ttattatact ggccttagca aatgcaaaca gagatgaatt tctgactaaa ccagaggtag 8761 ccagaagaag cagaataact aaaaatgtgc ctaaacaaag ctctcagctt gatgaggatc 8821 gaacagaggc tgcaaataga ataatatgta ctatcagaag taggagacct cagatggtca 8881 gaagtgttga ggcactttgt gatgcttata ttatattagc aaacttagat gccactcagt 8941 ggaagactca gagaaaaggc ataaatattc cagcagacca gccaattact aaacttaaga 9001 atttagaaga tgttgttgtc cctactatgg aaattaaggt ggaccacaca ggagaatatg 9061 gaaatctggt gactatacag tcatttaaag cagaatttcg cttagcagga ggtgtaaatt 9121 taccaaaaat aatagattgt gtaggttccg atggcaagga gaggagacag cttgttaagg 9181 gccgtgatga cctgagacaa gatgctgtca tgcaacaggt cttccagatg tgtaatacat 9241 tactgcagag aaacacggaa actaggaaga ggaaattaac tatctgtact tataaggtgg 9301 ttcccctctc tcagcgaagt ggtgttcttg aatggtgcac aggaactgtc cccattggtg 9361 aatttcttgt taacaatgaa gatggtgctc ataaaagata caggccaaat gatttcagtg 9421 cctttcagtg ccaaaagaaa atgatggagg tgcaaaaaaa gtcttttgaa gagaaatatg 9481 aagtcttcat ggatgtttgc caaaattttc aaccagtttt ccgttacttc tgcatggaaa 9541 aattcttgga tccagctatt tggtttgaga agcgattggc ttatacgcgc agtgtagcta 9601 cttcttctat tgttggttac atacttggac ttggtgatag acatgtacag aatatcttga 9661 taaatgagca gtcagcagaa cttgtacata tagatctagg tgttgctttt gaacagggca 9721 aaatccttcc tactcctgag acagttcctt ttagactcac cagagatatt gtggatggca 9781 tgggcattac gggtgttgaa ggtgtcttca gaagatgctg tgagaaaacc atggaagtga 9841 tgagaaactc tcaggaaact ctgttaacca ttgtagaggt ccttctatat gatccactct 9901 ttgactggac catgaatcct ttgaaagctt tgtatttaca gcagaggccg gaagatgaaa 9961 ctgagcttca ccctactctg aatgcagatg accaagaatg caaacgaaat ctcagtgata 10021 ttgaccagag tttcaacaaa gtagctgaac gtgtcttaat gagactacaa gagaaactga 10081 aaggagtgga agaaggcact gtgctcagtg ttggtggaca agtgaatttg ctcatacagc 10141 aggccataga ccccaaaaat ctcagccgac ttttcccagg atggaaagct tgggtgtgat 10201 cttcagtata tgaattaccc tttcattcag cctttagaaa ttatatttta gcctttattt 10261 ttaacctgcc aacatacttt aagtagggat taatatttaa gtgaactatt gtgggttttt 10321 ttgaatgttg gttttaatac ttgatttaat caccactcaa aaatgttttg atggtcttaa 10381 ggaacatctc tgctttcact ctttagaaat aatggtcatt cgggctgggc gcagcggctc 10441 acgcctgtaa tcccagcact ttgggaggcc gaggtgagcg gatcacaagg tcaggagttc 10501 gagaccagcc tggccaagag accagcctgg ccagtatggt gaaaccctgt ctctactaaa 10561 aatacaaaaa ttagccgagc atggtggcgg gcacctgtag tcccagctac tcgagcatgc 10621 atctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga 10681 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc 10741 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc 10801 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt 10861 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa 10921 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg 10981 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 11041 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 11101 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 11161 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 11221 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 11281 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt 11341 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca 11401 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 11461 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 11521 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 11581 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 11641 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt 11701 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga 11761 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc 11821 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg 11881 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac 11941 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc 12001 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg 12061 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga 12121 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg 12181 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg 12241 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt 12301 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc 12361 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg 12421 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga 12481 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc 12541 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc 12601 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc 12661 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag 12721 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat 12781 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 12841 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc 12901 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 12961 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 13021 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 13081 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 13141 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 13201 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 13261 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 13321 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 13381 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 13441 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 13501 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 13561 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 13621 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 13681 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 13741 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag 13801 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 13861 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 13921 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 13981 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 14041 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 14101 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 14161 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 14221 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 14281 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 14341 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 14401 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 14461 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 14521 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 14581 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 14641 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 14701 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 14761 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 14821 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 14881 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 14941 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 15001 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 15061 ccccgaaaag tgccacctga cgtc //
